This group focuses on the applications of enzymes, enzyme inhibitors, nanomaterials and biosensors in the food, health, biotech and environmental areas. The focus and problems being addressed are described below.

Improving the Thermostability of Maltogenic Alpha Amylase of Aspergillus Niger by using Negative Pressure

Maltogenic alpha amylase is helpful in the production of maltose from starch. But, this enzyme is stable only up to 40°C limiting its usage in the processing of starch. Calcium can stabilize the enzyme up to 50°C and can be further be improved upto 70°C by using negative pressure

  • Improving the Activities of Pectinase and Partially Saturated Canthaxanthin (PSC) Produced by Aspergillus Carbonarius by using Nano approaches

Pectinase has been used for the fruit juice clearance and oil extraction. Partially saturated canthaxanthin, used as food colour, showed anticancer and antioxidant activities. When both metabolites were treated with carbon nanotubes (CNTs), they demonstrated significantly high level of activities.

  • Enhancing the Functionality of Methotrexate (MTX) Degrading Enzyme from VariovoraxParadoxus by using Nano approaches

Methotrexate degrading enzymes are required to overcome the toxicity of methotrexate while treating cancer. The enzyme from Variovoraxparadoxus converts the methotrexate into non-toxic products. Methotrexate degrading enzyme from V. paradoxus is a dimeric protein with a molecular mass of 46 KDa. Enzyme nanoparticles can degrade methotrexate faster than the native enzyme and show lower Km compared to that of the native enzyme. Enzyme nanoparticles show better thermal stability and are found to be stable for much longer time in the serum compared to the native enzyme. They show better functionality than the native enzyme while clearing the methotrexate added to the serum,indicating their advantage over the native enzyme for therapeutic and biotechnological applications.

Identification and Isolation of Lipase Inhibitors from Streptomyces Spp.

Orlistat is the only commercially available lipase inhibitor for the treatment of obesity. But, it has side effects like gastric problems, acute kidney injury and acuteoxalate nephropathy. Therefore, there is a need to explore new inhibitors. Streptomyces spp (Fig.4) are being explored for inhibitors and new autobiographic methods have been developed (Fig.5) to detect lipase inhibitors.

Bioremediation of Toxic Textile Dyes by using Bilirubin Oxidase Enzyme

A major contributor of water pollution is textile dyes; some of them are carcinogenic. It has been found that bilirubin oxidase enzyme from the fungus Myrothecium verrucaria can degrade the textile dye reactive blue 25 significantly suggesting the usage of this enzyme for polluted water remediation.


Development of Enzyme Nano-biosensor for the Detection of Ammonium

Ammonia is one of the toxic contaminants of air, water and food. It is injurious to health.Its continuous monitoring is important to avoid tragedies. An ammonia biosensor based on the enzyme, alanine dehydrogenase, has been developed at the lab. The enzyme is highly specific and responds to a broad range of ammonium concentrations. Multi walled carbon nanotubes have also been found to enhance the response and sensitivity of the ammonia biosensor.

  1. Bayineni VK, Kadeppagari RK. 2021.Evaluation of p-nitrophenyl butyrate based bioautographic method for the identification of lipase inhibitors from the unexplored plant resources. Journal of Plant Biochemistry and Biotechnology. https://doi.org/10.1007/s13562-021-00686-w(IF: 1.23).
  2. Dave UC, Kadeppagari RK. 2019. Alanine dehydrogenase and its applications. Critical reviews in Biotechnology. 39: 648-664 (IF: 5.239)
  3. Dave UC, Kadeppagari RK. 2018. Purification and characterization of Alanine dehydrogenase from Streptomyces anulatus for its application as a bioreceptor in biosensor. Process Biochemistry. 68: 73-82(IF: 2.96)
  4. Bayineni VK, Suresh S , Sharma A, KadeppagariRK. 2018. Improvement of bilirubin oxidase productivity of Myrothecium verrucaria and studies on the enzyme overproduced by the mutant strain in the solid state fermentation. The Journal of General and Applied Microbiology. 64:68-75 (IF: 0.9)
  5. Bayineni VK, Venkatesh K, Sahu CK, KadeppagariRK. 2016. The 46 kDa dimeric protein from Variovoraxparadoxus shows faster methotrexate degrading activity in its nanoform compare to the native enzyme.Enzyme and Microbial Technology. 85:38-43 (IF: 3.55)

Biotechnology for Zero Waste (ISBN 978-3-527-34898-5). 2020. Editors-Hussain CM,Kadeppagari RK, Wiley-VCH, in press

Book Chapters

  1.  Balan A, Kadeppagari RK. Biopolymers for nano-enabled packaging of food. 2020. Handbook of Polymer and Ceramic Nanotechnology, Edited by HussainChaudheryMustansar and ThomasSabu, Springer, ISBN 978-3-030-40512-0. In press
  2. Banerjee S, Chandra Prakash P and Kadeppagari RK. Zein based nano-products in nutrition and food sectors. 2020. Handbook of Consumer Nano Products, Edited by ShadpourMallakpour and ChaudheryMustansar Hussain,Springer,In press. https://doi.org/10.1007/978-981-15-6453-6_51-1
  3. Rakshith KS, Kadeppagari RK. Functionalized carbon nanotubes for ammonia sensors. 2020. Environmental Applications of Carbon Nano-materials Based Devices. Edited by ShadpourMallakpour and ChaudheryMustansar Hussain, Wiley-VCH,In press
  4. Krishna Bayineni V., Naira V.R., Kadeppagari RK. (2021) Biomedical Applications of Nanozymes: Disease Diagnosis and Therapy. In: Handbook of Consumer Nanoproducts. Springer, Singapore. https://doi.org/10.1007/978-981-15-6453-6_91-1
  5. Kumar Sahu C., Dhanashri Sanjay S., Kadeppagari RK. (2021) Application of Nanotechnology for Encapsulation of Micronutrients. In: Handbook of Consumer Nanoproducts. Springer, Singapore. https://doi.org/10.1007/978-981-15-6453-6_93-1
  6. Rastogi M., Bhavana CV, Kadeppagari RK. (2021). Nanotechnology: A revolutionary approach toward food packaging. In: Handbook of Consumer Nanoproducts. Springer, Singapore. https://doi.org/10.1007/978-981-15-6453-6_92-1

Freeze dryer

-800 and -200C Freezers

Tip Sonicator

Electrophoresis Unit

Rotary Evaporator

Cooling Centrifuge

BOD Incubator

Laminar Air Flow

  1. Improvement of thermostability and functionality of cancer therapy related enzyme isolated from Variovoraxparadoxus by using nano approaches- VGST, Karnataka
  2. Nano CLEAs (cross-linked enzyme aggregates) for biomedical, food, agri and environmental applications-SSPS
  3. Ionizing radiations for improving the shelf life of fruits by delayed ripening-SSPS
  4. Development of nanobiosensors for the detection of food adulterants and health related metabolites or agents-SSPS
  5. Lipase inhibitors and their applications in food and health sectors-SSPS
  6. Solid state fermentation for the production of novel metabolites from fungi-SSPS
  7. Remediation of hazardous dyes by integrating biotechnological methods with radiations- SSPS
  8. Improving the functionalities of food and health related metabolites by using nano approaches-SSPS

Ravi Kumar Kadeppagari

Ravi Kumar Kadeppagari obtained his education from premier institutes of India, such as Regional Institute of Education (constituent of NCERT), Pondicherry Central University and Central Food Technological Research Institute (constituent of CSIR). He has overseas postdoctoral experience at Northwestern University, Chicago, and LSU Health Sciences Center, New Orleans, USA. He was a recipient of the Young Scientist Research Grant from DST- SERB, New Delhi, and has completed projects from DRDO, DST, VGST and MOFPI. He played a major role in setting up a Food Technology Division at Jain University by obtaining an infrastructural grant from Ministry of Food Processing Industries, New Delhi. He was the Editorial Board Member of American Journal of Bioscience & Bioengineering and the Associate Editor of Journal of Analytical & Pharmaceutical Research. He is an active reviewer for SCI/SCOPUS indexed international journals such as Starch, Current Microbiology, International Journal of Food Science & Technology, Journal of Separation Science, Journal of Chromatography-B, International Journal of Pharmacy & Pharmaceutical Sciences, Phytochemical Analysis, Journal of Electroanalytical Chemistry, FEMS Microbiology Letters, etc. He is a biotechnology consultant at international consulting agencies such as ‘Zintro’ and ‘Acabiz’. He is also a mentor for start-ups.

He has research publications in Scopus and SCI indexed international journals and patents to his credit. He has also supervised doctoral and master students, and is a life member of the Society of Biological Chemists, Association of Microbiologists of India, Indian Science Congress Association, Luminescence Society of India, Institute of Smart Structures & Systems, Association of Food Scientists & Technologists (India), and Society for Biotechnologists (India). He served as the Chairman of Board of Examiners, Food Technology, Jain University. He received the achievement award for his research contributions from Jain University Trust. He has also received junior and senior research fellowships from CSIR, New Delhi. He currently works as Professor at the Centre. His current research focuses on applications of enzymes, enzyme inhibitors, nanomaterials and biosensors in the food, health, biotech and environmental areas.

Email Id: ravikumar.k@ciirc.jyothyit.ac.in